› 5ev2

X-ray diffraction
1.86Å resolution

Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal

Released:
DOI: 10.2210/pdb5ev2/pdb
PDB entry 5ev2 coloured by chain, front view.
PDB entry 5ev2 coloured by chain, side view.
PDB entry 5ev2 coloured by chain, top view.
Source organisms:
Primary publication:
An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.
Agrawal AA, Salsi E, Chatrikhi R, Henderson S, Jenkins JL, Green MR, Ermolenko DN, Kielkopf CL
Nat Commun 7 10950 (2016)
PMID: 26952537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113175 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 141-341; Coverage: 42%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
DNA (5'-R(P*UP*U)-D(P*UP*U)-R(P*U)-D(P*UP*(BRU)P*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand DIO 1 x DIO
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
1 modified residue:
Ligand BRU 1 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2221
Unit cell:
a: 61.469Å b: 115.22Å c: 59.544Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.151 0.188
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Alternative-splicing defects in cancer: Splicing regulators and their downstream targets, guiding the way to novel cancer therapeutics.
Urbanski et al. (2018)
8 more

2 mentions without citation

An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.
Agrawal et al. (2016)
1 more