› 5ev4

X-ray diffraction
1.57Å resolution

Structure IV of Intact U2AF65 Recognizing the 3' Splice Site Signal

Released:
DOI: 10.2210/pdb5ev4/pdb
PDB entry 5ev4 coloured by chain, front view.
PDB entry 5ev4 coloured by chain, side view.
PDB entry 5ev4 coloured by chain, top view.
Source organisms:
Primary publication:
An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.
Agrawal AA, Salsi E, Chatrikhi R, Henderson S, Jenkins JL, Green MR, Ermolenko DN, Kielkopf CL
Nat Commun 7 10950 (2016)
PMID: 26952537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113177 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26368 (Residues: 141-341; Coverage: 42%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
DNA/RNA (5'-R(P*UP*UP*UP*(UD)P*UP*U)-D(P*(BRU)P*(UD))-R(P*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand BRU 1 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 43.502Å b: 63.411Å c: 77.662Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.176
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Alternative-splicing defects in cancer: Splicing regulators and their downstream targets, guiding the way to novel cancer therapeutics.
Urbanski et al. (2018)
8 more

5 mentions without citation

An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.
Agrawal et al. (2016)
4 more