5ewz

X-ray diffraction
2.34Å resolution

Small-molecule stabilization of the 14-3-3/Gab2 PPI interface

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-158988 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
GRB2-associated-binding protein 2 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQC2 (Residues: 387-395; Coverage: 1%)
Gene names: GAB2, KIAA0571
GRB2-associated-binding protein 2 Chain: D
Molecule details ›
Chain: D
Length: 6 amino acids
Theoretical weight: 735 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQC2 (Residues: 207-212; Coverage: 1%)
Gene names: GAB2, KIAA0571

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 73.66Å b: 104.92Å c: 116.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.244
Expression systems:
  • Escherichia coli
  • Not provided