› 5ez0

X-ray diffraction
2.35Å resolution

CRYSTAL STRUCTURE OF THE PTPN4 PDZ DOMAIN COMPLEXED WITH THE PDZ BINDING MOTIF OF THE MITOGEN ACTIVATED PROTEIN KINASE P38GAMMA.

Released:
DOI: 10.2210/pdb5ez0/pdb
PDB entry 5ez0 coloured by chain, front view.
PDB entry 5ez0 coloured by chain, side view.
PDB entry 5ez0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38γ.
Maisonneuve P, Caillet-Saguy C, Vaney MC, Bibi-Zainab E, Sawyer K, Raynal B, Haouz A, Delepierre M, Lafon M, Cordier F, Wolff N
J Biol Chem 291 16699-708 (2016)
PMID: 27246854

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110222 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29074 (Residues: 499-604; Coverage: 11%)
Gene name: PTPN4
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Protein kinase domain-containing protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: B5MDL5 (Residues: 269-277; Coverage: 3%)
Gene names: MAPK12, hCG_2039437

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 68.564Å b: 76.121Å c: 198.676Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Proteinaceous Regulators and Inhibitors of Protein Tyrosine Phosphatases.
Hendriks et al. (2018)
11 other articles

2 mentions without citation

Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38γ.
Maisonneuve et al. (2016)
1 more