› 5ez6

X-ray diffraction
1.8Å resolution

Crystallization and preliminary X-ray crystallographic analysis of a small GTPase RhoA

Released:
DOI: 10.2210/pdb5ez6/pdb
PDB entry 5ez6 coloured by chain, front view.
PDB entry 5ez6 coloured by chain, side view.
PDB entry 5ez6 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Yan Z, Ma S, Zhang Y, Ma L, Wang F, Li J, Miao L

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: B
Molecule details ›
Chain: B
Length: 181 amino acids
Theoretical weight: 20.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P43212
Unit cell:
a: 91.407Å b: 91.407Å c: 56.202Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.213
Expression system: Escherichia coli BL21(DE3)

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