› 5ezd

X-ray diffraction
2.1Å resolution

Crystal structure of a Hepatocyte growth factor activator inhibitor-1 (HAI-1) fragment covering the PKD-like 'internal' domain and Kunitz domain 1

Released:
DOI: 10.2210/pdb5ezd/pdb
PDB entry 5ezd coloured by chain, front view.
PDB entry 5ezd coloured by chain, side view.
PDB entry 5ezd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of a Two-domain Fragment of Hepatocyte Growth Factor Activator Inhibitor-1: FUNCTIONAL INTERACTIONS BETWEEN THE KUNITZ-TYPE INHIBITOR DOMAIN-1 AND THE NEIGHBORING POLYCYSTIC KIDNEY DISEASE-LIKE DOMAIN.
Hong Z, De Meulemeester L, Jacobi A, Pedersen JS, Morth JP, Andreasen PA, Jensen JK
J Biol Chem 291 14340-14355 (2016)
PMID: 27189939

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kunitz-type protease inhibitor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.78 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: O43278 (Residues: 168-303; Coverage: 28%)
Gene names: HAI1, SPINT1, UNQ223/PRO256
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 57.65Å b: 75.14Å c: 75.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.257
Expression system: Komagataella phaffii CBS 7435

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Citations

1 review citation

Priming of SARS-CoV-2 S protein by several membrane-bound serine proteinases could explain enhanced viral infectivity and systemic COVID-19 infection.
Fuentes-Prior P. (2021)
7 other articles

2 mentions without citation

Crystal Structure of a Two-domain Fragment of Hepatocyte Growth Factor Activator Inhibitor-1: FUNCTIONAL INTERACTIONS BETWEEN THE KUNITZ-TYPE INHIBITOR DOMAIN-1 AND THE NEIGHBORING POLYCYSTIC KIDNEY DISEASE-LIKE DOMAIN.
Hong et al. (2016)
1 more