› 5f0x

X-ray diffraction
1.6Å resolution

Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with 2'-deoxy-ADP and inorganic phosphate

Released:
DOI: 10.2210/pdb5f0x/pdb
PDB entry 5f0x coloured by chain, front view.
PDB entry 5f0x coloured by chain, side view.
PDB entry 5f0x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Probing the ATP Site of GRP78 with Nucleotide Triphosphate Analogs.
Hughes SJ, Antoshchenko T, Chen Y, Lu H, Pizarro JC, Park HW
PLoS One 11 e0154862 (2016)
PMID: 27144892

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoplasmic reticulum chaperone BiP Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11021 (Residues: 26-407; Coverage: 60%)
Gene names: GRP78, HSPA5
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
Ligand DTP 2 x DTP
Ligand DAT 1 x DAT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 56.347Å b: 74.448Å c: 85.882Å
α: 90° β: 97.67° γ: 90°
R-values:
R R work R free
0.176 0.174 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The endoplasmic reticulum (ER) chaperone BiP is a master regulator of ER functions: Getting by with a little help from ERdj friends.
Pobre et al. (2019)
3 more

2 mentions without citation

Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress.
Chipurupalli et al. (2022)
1 more