› 5f58

X-ray diffraction
1.54Å resolution

Crystal structure of the Q108K:K40L:T51V:R58F mutant of human Cellular Retinol Binding Protein II in complex with All-trans-Retinal after 24 hours of incubation at 1.54 Angstrom Resolution

Released:
DOI: 10.2210/pdb5f58/pdb
PDB entry 5f58 coloured by chain, front view.
PDB entry 5f58 coloured by chain, side view.
PDB entry 5f58 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Nosrati M, Nossoni Z, Geiger JH

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 30.234Å b: 35.704Å c: 64.104Å
α: 91.28° β: 90.91° γ: 114.19°
R-values:
R R work R free
0.195 0.193 0.229
Expression system: Escherichia coli BL21

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