5f5p

X-ray diffraction
3.57Å resolution

Molecular Basis for Shroom2 Recognition by Rock1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-171701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Shroom2 Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 217 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13796 (Residues: 1427-1610; Coverage: 11%)
Gene names: APXL, SHROOM2
Sequence domains: Apx/Shroom domain ASD2
Rho-associated protein kinase 1 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 86 amino acids
Theoretical weight: 10.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13464 (Residues: 834-913; Coverage: 6%)
Gene name: ROCK1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 97.587Å b: 133.795Å c: 135.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.271 0.287
Expression system: Escherichia coli BL21(DE3)