› 5faf

X-ray diffraction
1.05Å resolution

N184K pathological variant of gelsolin domain 2 (orthorhombic form)

Released:
DOI: 10.2210/pdb5faf/pdb
PDB entry 5faf coloured by chain, front view.
PDB entry 5faf coloured by chain, side view.
PDB entry 5faf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant.
Bonì F, Milani M, Porcari R, Barbiroli A, Ricagno S, de Rosa M
Sci Rep 6 33463 (2016)
PMID: 27633054

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gelsolin Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 178-293; Coverage: 15%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand GOL 3 x GOL
Ligand CL 2 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Unit cell:
a: 26.39Å b: 50.12Å c: 80.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.13 0.168
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Analyses Mutations in GSN, CST3, TTR, and ITM2B Genes in Chinese Patients With Alzheimer's Disease.
Jiang et al. (2020)
6 more

2 mentions without citation

Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein.
Bonì et al. (2018)
1 more