› 5fcn

X-ray diffraction
1.8Å resolution

microtubule binding domain of human CEP135

Released:
DOI: 10.2210/pdb5fcn/pdb
PDB entry 5fcn coloured by chain, front view.
PDB entry 5fcn coloured by chain, side view.
PDB entry 5fcn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding.
Kraatz S, Guichard P, Obbineni JM, Olieric N, Hatzopoulos GN, Hilbert M, Sen I, Missimer J, Gönczy P, Steinmetz MO
Structure 24 1358-1371 (2016)
PMID: 27477386

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centrosomal protein of 135 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q66GS9 (Residues: 82-144; Coverage: 6%)
Gene names: CEP135, CEP4, KIAA0635

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 78.85Å b: 78.85Å c: 95.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The genetic architecture of morphological abnormalities of the sperm tail.
Touré et al. (2021)
8 more

1 mention without citation

Molecular Modeling the Proteins from the exo-xis Region of Lambda and Shigatoxigenic Bacteriophages.
Donaldson LW. (2021)