› 5fd3

X-ray diffraction
2.42Å resolution

Structure of Lin54 tesmin domain bound to DNA

Released:
DOI: 10.2210/pdb5fd3/pdb
PDB entry 5fd3 coloured by chain, front view.
PDB entry 5fd3 coloured by chain, side view.
PDB entry 5fd3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters.
Marceau AH, Felthousen JG, Goetsch PD, Iness AN, Lee HW, Tripathi SM, Strome S, Litovchick L, Rubin SM
Nat Commun 7 12301 (2016)
PMID: 27465258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118669 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein lin-54 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6MZP7 (Residues: 515-646; Coverage: 18%)
Gene names: CXCDC1, KIAA2037, LIN54
Sequence domains: Tesmin/TSO1-like CXC domain, cysteine-rich domain
DNA (5'-D(*GP*AP*GP*TP*TP*TP*GP*AP*AP*AP*CP*T)-3') Chains: C, H
Molecule details ›
Chains: C, H
Length: 12 nucleotides
Theoretical weight: 3.7 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*GP*TP*TP*TP*CP*AP*AP*AP*CP*TP*C)-3') Chains: D, I
Molecule details ›
Chains: D, I
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 39.32Å b: 53.88Å c: 67.12Å
α: 98.4° β: 102.53° γ: 106.57°
R-values:
R R work R free
0.184 0.181 0.238
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

5 review citations

Cell cycle arrest through indirect transcriptional repression by p53: I have a DREAM.
Engeland K. (2018)
4 more

4 mentions without citation

Structure and function of MuvB complexes.
Müller et al. (2022)
3 more