› 5ffw

X-ray diffraction
1.5Å resolution

Crystal structure of the bromodomain of human BRPF1 in complex with H4K5acK8ac histone peptide

Released:
DOI: 10.2210/pdb5ffw/pdb
PDB entry 5ffw coloured by chain, front view.
PDB entry 5ffw coloured by chain, side view.
PDB entry 5ffw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tallant C, Nunez-Alonso G, Savitsky P, Krojer T, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Knapp S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157225 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peregrin Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55201 (Residues: 626-740; Coverage: 10%)
Gene names: BR140, BRPF1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H4 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P62805 (Residues: 2-11; Coverage: 10%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 87.348Å b: 93.177Å c: 32.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.243
Expression systems:
  • Escherichia coli
  • Not provided

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