› 5fhx

X-ray diffraction
2.55Å resolution

CRYSTAL STRUCTURE OF CODV IN COMPLEX WITH IL4 AT 2.55 Ang. RESOLUTION.

Released:
DOI: 10.2210/pdb5fhx/pdb
PDB entry 5fhx coloured by chain, front view.
PDB entry 5fhx coloured by chain, side view.
PDB entry 5fhx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CODV-Ig, a universal bispecific tetravalent and multifunctional immunoglobulin format for medical applications.
Steinmetz A, Vallée F, Beil C, Lange C, Baurin N, Beninga J, Capdevila C, Corvey C, Dupuy A, Ferrari P, Rak A, Wonerow P, Kruip J, Mikol V, Rao E
MAbs 8 867-78 (2016)
PMID: 26984268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209233 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-4 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05112 (Residues: 30-153; Coverage: 96%)
Gene name: IL4
Sequence domains: Interleukin 4
Structure domains: Growth Hormone; Chain: A;
antibody fragment heavy-chain Chain: H
Molecule details ›
Chain: H
Length: 345 amino acids
Theoretical weight: 37.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 334 amino acids
Theoretical weight: 36.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 77.21Å b: 73.33Å c: 91.4Å
α: 90° β: 112.72° γ: 90°
R-values:
R R work R free
0.187 0.184 0.249
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The making of bispecific antibodies.
Brinkmann et al. (2017)
8 more

4 mentions without citation

CODV-Ig, a universal bispecific tetravalent and multifunctional immunoglobulin format for medical applications.
Steinmetz et al. (2016)
3 more