› 5fhz

X-ray diffraction
2.9Å resolution

Human aldehyde dehydrogenase 1A3 complexed with NAD(+) and retinoic acid

Released:
DOI: 10.2210/pdb5fhz/pdb
PDB entry 5fhz coloured by chain, front view.
PDB entry 5fhz coloured by chain, side view.
PDB entry 5fhz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human aldehyde dehydrogenase 1A3 complexed with NAD+ and retinoic acid.
Moretti A, Li J, Donini S, Sobol RW, Rizzi M, Garavaglia S
Sci Rep 6 35710 (2016)
PMID: 27759097

Function and Biology Details

Reaction catalysed: 
Retinal + NAD(+) + H(2)O = retinoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinaldehyde dehydrogenase 3 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 529 amino acids
Theoretical weight: 58.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47895 (Residues: 1-512; Coverage: 100%)
Gene names: ALDH1A3, ALDH6
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
1 bound ligand:
Ligand REA 4 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 82.656Å b: 159.785Å c: 177.647Å
α: 90° β: 93.69° γ: 90°
R-values:
R R work R free
0.231 0.228 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Genetic architecture of retinoic-acid signaling-associated ocular developmental defects.
Nedelec et al. (2019)
3 more

15 mentions without citation

Insights into Aldehyde Dehydrogenase Enzymes: A Structural Perspective.
Shortall et al. (2021)
14 more