› 5fii

X-ray diffraction
1.8Å resolution

Structure of a human aspartate kinase, chorismate mutase and TyrA domain.

Released:
DOI: 10.2210/pdb5fii/pdb
PDB entry 5fii coloured by chain, front view.
PDB entry 5fii coloured by chain, side view.
PDB entry 5fii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ligand-dependent dimerization of phenylalanine hydroxylase regulatory domain.
Patel D, Kopec J, Fitzpatrick F, McCorvie TJ, Yue WW
Sci Rep 6 23748 (2016)
PMID: 27049649

Function and Biology Details

Reaction catalysed: 
L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine-4-hydroxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 102 amino acids
Theoretical weight: 11.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00439 (Residues: 19-118; Coverage: 22%)
Gene name: PAH
Sequence domains: ACT domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand PHE 4 x PHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 36.14Å b: 37.41Å c: 64.08Å
α: 98.94° β: 103.84° γ: 94.79°
R-values:
R R work R free
0.179 0.177 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Markov State Models to Elucidate Ligand Binding Mechanism.
Ge et al. (2021)
3 more

15 mentions without citation

From structural biology to designing therapy for inborn errors of metabolism.
Yue WW. (2016)
14 more