5fj4

X-ray diffraction
2.95Å resolution

Structure of the standard kink turn HmKt-7 as stem loop bound with U1A and L7Ae proteins

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-128132 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 102 amino acids
Theoretical weight: 11.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-102; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Large ribosomal subunit protein eL8 Chains: C, G
Molecule details ›
Chains: C, G
Length: 123 amino acids
Theoretical weight: 13.49 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29494 (Residues: 5-119; Coverage: 97%)
Gene names: AF_0764, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
HMKT-7 Chains: D, H
Molecule details ›
Chains: D, H
Length: 35 nucleotides
Theoretical weight: 11.31 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 131.853Å b: 161.226Å c: 149.899Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.237
Expression system: Escherichia coli BL21(DE3)