› 5fjv

X-ray diffraction
3.2Å resolution

Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly

Released:
DOI: 10.2210/pdb5fjv/pdb
PDB entry 5fjv coloured by chain, front view.
PDB entry 5fjv coloured by chain, side view.
PDB entry 5fjv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a human neuronal nAChR extracellular domain in pentameric assembly: Ligand-bound α2 homopentamer.
Kouvatsos N, Giastas P, Chroni-Tzartou D, Poulopoulou C, Tzartos SJ
Proc Natl Acad Sci U S A 113 9635-40 (2016)
PMID: 27493220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal acetylcholine receptor subunit alpha-2 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 213 amino acids
Theoretical weight: 25.09 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q15822 (Residues: 59-265; Coverage: 41%)
Gene name: CHRNA2
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand EPJ 5 x EPJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 101.524Å b: 116.559Å c: 129.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.224 0.308
Expression system: Komagataella pastoris

Quick links

Citations

11 review citations

Neuronal Nicotinic Acetylcholine Receptor Modulators from Cone Snails.
Abraham et al. (2018)
10 more

5 mentions without citation

Understanding structure-function relationships of the human neuronal acetylcholine receptor: insights from the first crystal structures of neuronal subunits.
Giastas et al. (2018)
4 more