› 5fk9

X-ray diffraction
3.1Å resolution

Crystal structure of staphylococcal enterotoxin A F47A mutant in complex with a T cell receptor

Released:
DOI: 10.2210/pdb5fk9/pdb
PDB entry 5fk9 coloured by chain, front view.
PDB entry 5fk9 coloured by chain, side view.
PDB entry 5fk9 coloured by chain, top view.
Source organisms:
Primary publication:
Two common structural motifs for TCR recognition by staphylococcal enterotoxins.
Rödström KEJ, Regenthal P, Bahl C, Ford A, Baker D, Lindkvist-Petersson K
Sci Rep 6 25796 (2016)
PMID: 27180909

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-100938 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
T cell receptor alpha chain constant; T cell receptor alpha variable 22 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B4J277 (Residues: 21-110; Coverage: 100%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC, TRAV22
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein; T cell receptor beta constant 2 Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5A3 (Residues: 20-115; Coverage: 99%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TCRBV6S4A1, TRBC2
Sequence domains:
Structure domains: Immunoglobulins
Enterotoxin type A Chain: C
Molecule details ›
Chain: C
Length: 233 amino acids
Theoretical weight: 27.05 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A0L2 (Residues: 25-257; Coverage: 100%)
Gene name: entA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 114.34Å b: 150.44Å c: 39.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.257 0.291
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12

Quick links

Citations

4 citation in other articles

Binding of Catechins to Staphylococcal Enterotoxin A.
Shimamura et al. (2018)
3 more

6 mentions without citation

Staphylococcal and Streptococcal Superantigens Trigger B7/CD28 Costimulatory Receptor Engagement to Hyperinduce Inflammatory Cytokines.
Popugailo et al. (2019)
5 more