› 5flc

Electron Microscopy
5.9Å resolution

Architecture of human mTOR Complex 1 - 5.9 Angstrom reconstruction

Released:
DOI: 10.2210/pdb5flc/pdb
PDB entry 5flc coloured by chain, front view.
PDB entry 5flc coloured by chain, side view.
PDB entry 5flc coloured by chain, top view.
Source organisms:
Primary publication:
Architecture of human mTOR complex 1.
Aylett CH, Sauer E, Imseng S, Boehringer D, Hall MN, Ban N, Maier T
Science 351 48-52 (2016)
PMID: 26678875
Related structures: EMD-3213

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-154629 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
SERINE/THREONINE-PROTEIN KINASE MTOR Chains: 1, 3
Molecule details ›
Chains: 1, 3
Length: 615 amino acids
Theoretical weight: 52.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
SERINE/THREONINE-PROTEIN KINASE MTOR Chains: 2, 4
Molecule details ›
Chains: 2, 4
Length: 365 amino acids
Theoretical weight: 31.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
REGULATORY-ASSOCIATED PROTEIN OF MTOR Chains: A, E
Molecule details ›
Chains: A, E
Length: 1029 amino acids
Theoretical weight: 87.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Serine/threonine-protein kinase mTOR Chains: B, F
Molecule details ›
Chains: B, F
Length: 1168 amino acids
Theoretical weight: 134.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42345 (Residues: 1382-2549; Coverage: 46%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
FKBP Chains: C, G
Molecule details ›
Chains: C, G
Length: 107 amino acids
Theoretical weight: 9.12 KDa
Source organism: Spodoptera frugiperda
Target of rapamycin complex subunit LST8 Chains: D, H
Molecule details ›
Chains: D, H
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

1 bound ligand:
Ligand RAP 2 x RAP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.9Å
Relevant EMDB volumes: EMD-3213
Expression system: Spodoptera frugiperda

Quick links

Citations

77 review citations

The Mechanistic Target of Rapamycin: The Grand ConducTOR of Metabolism and Aging.
Kennedy et al. (2016)
76 more

15 mentions without citation

mTOR Signaling in Growth, Metabolism, and Disease.
Saxton et al. (2017)
14 more