› 5fna

Electron Microscopy
4.8Å resolution

Cryo-EM reconstruction of caspase-1 CARD

Released:
DOI: 10.2210/pdb5fna/pdb
PDB entry 5fna coloured by chain, front view.
PDB entry 5fna coloured by chain, side view.
PDB entry 5fna coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism.
Lu A, Li Y, Schmidt FI, Yin Q, Chen S, Fu TM, Tong AB, Ploegh HL, Mao Y, Wu H
Nat. Struct. Mol. Biol. (2016)
PMID: 27043298
Related structures: EMD-3241

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 85 amino acids
Theoretical weight: 9.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29466 (Residues: 2-86; Coverage: 21%)
Gene names: CASP1, IL1BC, IL1BCE
Sequence domains: Caspase recruitment domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-3241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Orexin/Hypocretin Signaling.
Kukkonen JP. (2017)
39 more

15 mentions without citation

Therapeutic implications of inflammasome in inflammatory bowel disease.
Khatri et al. (2021)
14 more