› 5foe

X-ray diffraction
1.98Å resolution

Crystal structure of the C. elegans Protein O-fucosyltransferase 2 (CePOFUT2) double mutant (R298K-R299K) in complex with GDP and the human TSR1 from thrombospondin 1

Released:
DOI: 10.2210/pdb5foe/pdb
PDB entry 5foe coloured by chain, front view.
PDB entry 5foe coloured by chain, side view.
PDB entry 5foe coloured by chain, top view.
Source organisms:
Primary publication:
A proactive role of water molecules in acceptor recognition by protein O-fucosyltransferase 2.
Valero-González J, Leonhard-Melief C, Lira-Navarrete E, Jiménez-Osés G, Hernández-Ruiz C, Pallarés MC, Yruela I, Vasudevan D, Lostao A, Corzana F, Takeuchi H, Haltiwanger RS, Hurtado-Guerrero R
Nat Chem Biol 12 240-6 (2016)
PMID: 26854667

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-fucose protein O-fucosyltransferase 2; Thrombospondin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 500 amino acids
Theoretical weight: 57.52 KDa
Source organisms: Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8WR51 (Residues: 1-424; Coverage: 100%)
  • Canonical: P07996 (Residues: 378-429; Coverage: 5%)
Gene names: K10G9.3, THBS1, TSP, TSP1, pad-2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand GDP 2 x GDP
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 88.026Å b: 67.447Å c: 90.411Å
α: 90° β: 116.07° γ: 90°
R-values:
R R work R free
0.202 0.2 0.246
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Biological functions of fucose in mammals.
Schneider et al. (2017)
5 more

10 mentions without citation

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)
9 more