› 5fsk

X-ray diffraction
1.56Å resolution

MTH1 substrate recognition: Complex with 8-oxo-dGTP.

Released:
DOI: 10.2210/pdb5fsk/pdb
PDB entry 5fsk coloured by chain, front view.
PDB entry 5fsk coloured by chain, side view.
PDB entry 5fsk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MTH1 Substrate Recognition--An Example of Specific Promiscuity.
Nissink JW, Bista M, Breed J, Carter N, Embrey K, Read J, Winter-Holt JJ
PLoS One 11 e0151154 (2016)
PMID: 26999531

Function and Biology Details

Reaction catalysed: 
2-hydroxy-dATP + H(2)O = 2-hydroxy-dAMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidized purine nucleoside triphosphate hydrolase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36639 (Residues: 1-156; Coverage: 100%)
Gene names: MTH1, NUDT1
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

2 bound ligands:
Ligand H6Y 1 x H6Y
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 60.495Å b: 65.608Å c: 36.348Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.242
Expression system: Escherichia coli

Quick links

Citations

2 review citations

8-Oxo-7,8-dihydroadenine and 8-Oxo-7,8-dihydroadenosine-Chemistry, Structure, and Function in RNA and Their Presence in Natural Products and Potential Drug Derivatives.
Choi et al. (2017)
1 more

1 mention without citation

MTH1 Substrate Recognition--An Example of Specific Promiscuity.
Nissink et al. (2016)