› 5fu6

X-ray diffraction
2.9Å resolution

NOT module of the human CCR4-NOT complex (Crystallization mutant)

Released:
DOI: 10.2210/pdb5fu6/pdb
PDB entry 5fu6 coloured by chain, front view.
PDB entry 5fu6 coloured by chain, side view.
PDB entry 5fu6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.
Raisch T, Bhandari D, Sabath K, Helms S, Valkov E, Weichenrieder O, Izaurralde E
EMBO J 35 974-90 (2016)
PMID: 26968986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107785 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CCR4-NOT transcription complex subunit 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 535 amino acids
Theoretical weight: 61.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YKK6 (Residues: 1833-2361; Coverage: 22%)
Gene names: AD-005, CDC39, CNOT1, KIAA1007, NOT1
Sequence domains: CCR4-Not complex component, Not1
CCR4-NOT transcription complex subunit 2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 197 amino acids
Theoretical weight: 22.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZN8 (Residues: 350-540; Coverage: 35%)
Gene names: CDC36, CNOT2, HSPC131, MSTP046, NOT2
Sequence domains: NOT2/NOT3/NOT5 C-terminal
Structure domains: CCR4-NOT complex subunit 2/3/5, C-terminal domain
CCR4-NOT transcription complex subunit 3 Chains: C, F
Molecule details ›
Chains: C, F
Length: 148 amino acids
Theoretical weight: 18.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75175 (Residues: 607-748; Coverage: 19%)
Gene names: CNOT3, KIAA0691, LENG2, NOT3
Sequence domains: NOT2/NOT3/NOT5 C-terminal
Structure domains: CCR4-NOT complex subunit 2/3/5, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 75.98Å b: 135.32Å c: 101.33Å
α: 90° β: 107.67° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.
Passmore et al. (2022)
9 more

1 mention without citation

Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.
Raisch et al. (2016)