› 5fuo

X-ray diffraction
3.6Å resolution

Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life

Released:
DOI: 10.2210/pdb5fuo/pdb
PDB entry 5fuo coloured by chain, front view.
PDB entry 5fuo coloured by chain, side view.
PDB entry 5fuo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Extending the half-life of a fab fragment through generation of a humanized anti-human serum albumin Fv domain: An investigation into the correlation between affinity and serum half-life.
Adams R, Griffin L, Compson JE, Jairaj M, Baker T, Ceska T, West S, Zaccheo O, Davé E, Lawson AD, Humphreys DP, Heywood S
MAbs 8 1336-1346 (2016)
PMID: 27315033

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208786 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Albumin Chain: A
Molecule details ›
Chain: A
Length: 585 amino acids
Theoretical weight: 66.57 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02768 (Residues: 25-609; Coverage: 99%)
Gene names: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341
Sequence domains: Serum albumin family
Structure domains: Serum Albumin; Chain A, Domain 1
FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 24.9 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 217.64Å b: 217.64Å c: 78.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.254
Expression system: Cricetulus griseus

Quick links

Citations

4 review citations

Overview of Antibody Drug Delivery.
Awwad et al. (2018)
3 more

5 mentions without citation

Non-covalent albumin-binding ligands for extending the circulating half-life of small biotherapeutics.
Zorzi et al. (2019)
4 more