› 5fwp

Electron Microscopy
7.2Å resolution

Atomic cryoEM structure of Hsp90-Cdc37-Cdk4 complex

Released:
DOI: 10.2210/pdb5fwp/pdb
PDB entry 5fwp coloured by chain, front view.
PDB entry 5fwp coloured by chain, side view.
PDB entry 5fwp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hsp90 traps and stabilizes an unfolded kinase.
Verba KA, Wang RY, Arakawa A, Liu Y, Shirouzu M, Yokoyama S, Agard DA
Science 352 1542-7 (2016)
PMID: 27339980
Related structures: EMD-3340

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-140050 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat shock protein HSP 90-beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 727 amino acids
Theoretical weight: 83.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08238 (Residues: 1-724; Coverage: 100%)
Gene names: HSP90AB1, HSP90B, HSPC2, HSPC3, HSPCB
Sequence domains:
Hsp90 co-chaperone Cdc37 Chain: E
Molecule details ›
Chain: E
Length: 378 amino acids
Theoretical weight: 44.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16543 (Residues: 1-378; Coverage: 100%)
Gene names: CDC37, CDC37A
Sequence domains:
Cyclin-dependent kinase 4 Chain: K
Molecule details ›
Chain: K
Length: 310 amino acids
Theoretical weight: 34.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11802 (Residues: 1-303; Coverage: 100%)
Gene name: CDK4
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 7.2Å
Relevant EMDB volumes: EMD-3340
Expression system: Spodoptera frugiperda

Quick links

Citations

42 review citations

The HSP90 chaperone machinery.
Schopf et al. (2017)
41 more

10 mentions without citation

The disruption of protein-protein interactions with co-chaperones and client substrates as a strategy towards Hsp90 inhibition.
Serwetnyk et al. (2021)
9 more