› 5ggn

X-ray diffraction
1.21Å resolution

Crystal structure of N-terminal domain of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with GlcNAc-beta-pNP

Released:
DOI: 10.2210/pdb5ggn/pdb
PDB entry 5ggn coloured by chain, front view.
PDB entry 5ggn coloured by chain, side view.
PDB entry 5ggn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Carbohydrate-binding domain of the POMGnT1 stem region modulates O-mannosylation sites of α-dystroglycan.
Kuwabara N, Manya H, Yamada T, Tateno H, Kanagawa M, Kobayashi K, Akasaka-Manya K, Hirose Y, Mizuno M, Ikeguchi M, Toda T, Hirabayashi J, Senda T, Endo T, Kato R
Proc Natl Acad Sci U S A 113 9280-5 (2016)
PMID: 27493216

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZA1 (Residues: 92-250; Coverage: 24%)
Gene names: MGAT1.2, POMGNT1, UNQ746/PRO1475
Sequence domains: Interleukin-like EMT inducer

Ligands and Environments

1 bound ligand:
Ligand LEC 2 x LEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P21212
Unit cell:
a: 70.524Å b: 90.481Å c: 53.634Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.144 0.166
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)
9 more

2 mentions without citation

Bacteriophage T4 long tail fiber domains.
Hyman et al. (2018)
1 more