› 5glf

X-ray diffraction
2.25Å resolution

Structural insights into the interaction of p97 N-terminal domain and SHP motif in Derlin-1 rhomboid pseudoprotease

Released:
DOI: 10.2210/pdb5glf/pdb
PDB entry 5glf coloured by chain, front view.
PDB entry 5glf coloured by chain, side view.
PDB entry 5glf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the interaction of human p97 N-terminal domain and SHP motif in Derlin-1 rhomboid pseudoprotease.
Lim JJ, Lee Y, Yoon SY, Ly TT, Kang JY, Youn HS, An JY, Lee JG, Park KR, Kim TG, Yang JK, Jun Y, Eom SH
FEBS Lett 590 4402-4413 (2016)
PMID: 27714797

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transitional endoplasmic reticulum ATPase Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 184 amino acids
Theoretical weight: 20.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55072 (Residues: 21-199; Coverage: 22%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
Structure domains:
Derlin-1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BUN8 (Residues: 239-250; Coverage: 5%)
Gene names: DER1, DERL1, UNQ243/PRO276

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 43.802Å b: 74.402Å c: 222.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The AAA+ ATPase p97, a cellular multitool.
Stach et al. (2017)
1 more

3 mentions without citation

The Interplay of Cofactor Interactions and Post-translational Modifications in the Regulation of the AAA+ ATPase p97.
Hänzelmann et al. (2017)
2 more