› 5gox

X-ray diffraction
2.4Å resolution

Eukaryotic Rad50 Functions as A Rod-shaped Dimer

Released:
DOI: 10.2210/pdb5gox/pdb
PDB entry 5gox coloured by chain, front view.
PDB entry 5gox coloured by chain, side view.
PDB entry 5gox coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Eukaryotic Rad50 functions as a rod-shaped dimer.
Park YB, Hohl M, Padjasek M, Jeong E, Jin KS, Krężel A, Petrini JH, Cho Y
Nat Struct Mol Biol 24 248-257 (2017)
PMID: 28134932

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein RAD50 Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92878 (Residues: 585-766; Coverage: 14%)
Gene name: RAD50
Sequence domains: Rad50 zinc hook motif

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 42.18Å b: 61.972Å c: 81.54Å
α: 90° β: 99.75° γ: 90°
R-values:
R R work R free
0.216 0.213 0.271
Expression system: Escherichia coli

Quick links

Citations

6 review citations

CtIP/Ctp1/Sae2, molecular form fit for function.
Andres et al. (2017)
5 more

9 mentions without citation

A Survey of Reported Disease-Related Mutations in the MRE11-RAD50-NBS1 Complex.
Rahman et al. (2020)
8 more