› 5grj

X-ray diffraction
3.21Å resolution

Crystal structure of human PD-L1 with monoclonal antibody avelumab

Released:
DOI: 10.2210/pdb5grj/pdb
PDB entry 5grj coloured by chain, front view.
PDB entry 5grj coloured by chain, side view.
PDB entry 5grj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of anti-PD-L1 monoclonal antibody avelumab for tumor therapy.
Liu K, Tan S, Chai Y, Chen D, Song H, Zhang CW, Shi Y, Liu J, Tan W, Lyu J, Gao S, Yan J, Qi J, Gao GF
Cell Res 27 151-153 (2017)
PMID: 27573173

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235414 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
avelumab H chain Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
avelumab L chain Chain: L
Molecule details ›
Chain: L
Length: 110 amino acids
Theoretical weight: 11.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Programmed cell death 1 ligand 1 Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 25.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZQ7 (Residues: 18-238; Coverage: 81%)
Gene names: B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 120.87Å b: 98.151Å c: 62.036Å
α: 90° β: 108.43° γ: 90°
R-values:
R R work R free
0.205 0.204 0.238
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structural Biology of the Immune Checkpoint Receptor PD-1 and Its Ligands PD-L1/PD-L2.
Zak et al. (2017)
16 more

20 mentions without citation

The Diverse Function of PD-1/PD-L Pathway Beyond Cancer.
Qin et al. (2019)
19 more