› 5gs0

X-ray diffraction
3.28Å resolution

Crystal structure of the complex of TLR3 and bi-specific diabody

Released:
DOI: 10.2210/pdb5gs0/pdb
PDB entry 5gs0 coloured by chain, front view.
PDB entry 5gs0 coloured by chain, side view.
PDB entry 5gs0 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface.
Kim JH, Song DH, Youn SJ, Kim JW, Cho G, Kim SC, Lee H, Jin MS, Lee JO
Sci Rep 6 34515 (2016)
PMID: 27682821

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234985 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Toll-like receptor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 671 amino acids
Theoretical weight: 76.4 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O15455 (Residues: 27-697; Coverage: 76%)
Gene name: TLR3
Sequence domains:
Structure domains: Ribonuclease Inhibitor
light chain (anti-TLR3) Chains: C, E
Molecule details ›
Chains: C, E
Length: 107 amino acids
Theoretical weight: 11.49 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
heavy chain (anti-TLR3) Chains: D, F
Molecule details ›
Chains: D, F
Length: 127 amino acids
Theoretical weight: 13.86 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
heavy chain (anti-Lid) Chains: H, X
Molecule details ›
Chains: H, X
Length: 121 amino acids
Theoretical weight: 13.36 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
light chain (anti-Lid) Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 107 amino acids
Theoretical weight: 11.72 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand NAG 34 x NAG
Ligand BMA 4 x BMA
Ligand MAN 2 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 98.519Å b: 141.329Å c: 150.831Å
α: 90° β: 106.88° γ: 90°
R-values:
R R work R free
0.194 0.192 0.248
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Perspectives on metals-based radioimmunotherapy (RIT): moving forward.
White et al. (2021)
10 other articles

5 mentions without citation

Design an Efficient Multi-Epitope Peptide Vaccine Candidate Against SARS-CoV-2: An in silico Analysis.
Yazdani et al. (2020)
4 more