› 5gsq

X-ray diffraction
1.85Å resolution

Crystal structure of IgG Fc with a homogeneous glycoform and Antibody-Dependent Cellular Cytotoxicity

Released:
DOI: 10.2210/pdb5gsq/pdb
PDB entry 5gsq coloured by chain, front view.
PDB entry 5gsq coloured by chain, side view.
PDB entry 5gsq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of a Homogeneous IgG-Fc Glycoform with the N-Glycan Designed to Maximize the Antibody Dependent Cellular Cytotoxicity.
Chen CL, Hsu JC, Lin CW, Wang CH, Tsai MH, Wu CY, Wong CH, Ma C
ACS Chem Biol 12 1335-1345 (2017)
PMID: 28318221

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.34 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 106-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 50.183Å b: 158.406Å c: 66.905Å
α: 90° β: 109.04° γ: 90°
R-values:
R R work R free
0.229 0.227 0.267
Expression system: Cricetulus griseus

Quick links

Citations

10 review citations

Development of therapeutic antibodies for the treatment of diseases.
Lu et al. (2020)
9 more

2 mentions without citation

Enhanced Immunomodulatory Effect of Intravenous Immunoglobulin by Fc Galactosylation and Nonfucosylation.
Mimura et al. (2022)
1 more