› 5gvt

X-ray diffraction
2.61Å resolution

Crystal structures of the serine protease domain of murine plasma kallikrein

Released:
DOI: 10.2210/pdb5gvt/pdb
PDB entry 5gvt coloured by chain, front view.
PDB entry 5gvt coloured by chain, side view.
PDB entry 5gvt coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal Structures of the Serine Protease Domain of Murine Plasma Kallikrein
Xu M, Xu P, Zhou X, Andreasen P, Jiang L, Huang M
Chin. J. Struct. Chem. 36 242-249 (2017)

Function and Biology Details

Reaction catalysed: 
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma kallikrein light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 27.86 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P26262 (Residues: 391-638; Coverage: 40%)
Gene names: Klk3, Klkb1, Pk
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand BMA 2 x BMA
Ligand MAN 2 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 72.231Å b: 85.556Å c: 109.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.253
Expression system: Komagataella pastoris

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