› 5gw6

X-ray diffraction
1.97Å resolution

Water-Bridge Mediates Recognition of mRNA Cap in eIF4E

Released:
DOI: 10.2210/pdb5gw6/pdb
PDB entry 5gw6 coloured by chain, front view.
PDB entry 5gw6 coloured by chain, side view.
PDB entry 5gw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Water-Bridge Mediates Recognition of mRNA Cap in eIF4E.
Lama D, Pradhan MR, Brown CJ, Eapen RS, Joseph TL, Kwoh CK, Lane DP, Verma CS
Structure 25 188-194 (2017)
PMID: 27916520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 23-217; Coverage: 90%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 121.695Å b: 128.024Å c: 38.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Role of Cytoplasmic mRNA Cap-Binding Protein Complexes in Trypanosoma brucei and Other Trypanosomatids.
Freire et al. (2017)
1 more

1 mention without citation

PepPro: A Nonredundant Structure Data Set for Benchmarking Peptide-Protein Computational Docking.
Xu et al. (2020)