5gxw

X-ray diffraction
2.39Å resolution

Importin and NuMA complex

Released:
Source organisms:
Primary publication:
Regulation of mitotic spindle assembly factor NuMA by Importin-β.
J Cell Biol 216 3453-3462 (2017)
PMID: 28939615

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-156520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-497; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nuclear mitotic apparatus protein 1 Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14980 (Residues: 1984-2010; Coverage: 1%)
Gene names: NMP22, NUMA, NUMA1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 78.065Å b: 89.658Å c: 101.187Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.218
Expression system: Escherichia coli