› 5h1b

Electron Microscopy
4.4Å resolution

Human RAD51 presynaptic complex

Released:
DOI: 10.2210/pdb5h1b/pdb
PDB entry 5h1b coloured by chain, front view.
PDB entry 5h1b coloured by chain, side view.
PDB entry 5h1b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange.
Xu J, Zhao L, Xu Y, Zhao W, Sung P, Wang HW
Nat Struct Mol Biol 24 40-46 (2017)
PMID: 27941862
Related structures: EMD-9566

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118724 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA repair protein RAD51 homolog 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 339 amino acids
Theoretical weight: 37.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q06609 (Residues: 1-339; Coverage: 100%)
Gene names: RAD51, RAD51A, RECA
Sequence domains:
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.69 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 3 x ANP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-9566
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

14 review citations

Meiotic Recombination: Mixing It Up in Plants.
Wang et al. (2018)
13 more

15 mentions without citation

Structural basis of homologous recombination.
Sun et al. (2020)
14 more