› 5h2u

X-ray diffraction
2.24Å resolution

Crystal structure of PTK6 Kinase Domain complexed with Dasatinib

Released:
DOI: 10.2210/pdb5h2u/pdb
PDB entry 5h2u coloured by chain, front view.
PDB entry 5h2u coloured by chain, side view.
PDB entry 5h2u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Co-crystal structures of PTK6: With Dasatinib at 2.24 Å, with novel imidazo[1,2-a]pyrazin-8-amine derivative inhibitor at 1.70 Å resolution.
Thakur MK, Birudukota S, Swaminathan S, Battula SK, Vadivelu S, Tyagi R, Gosu R
Biochem Biophys Res Commun 482 1289-1295 (2017)
PMID: 27993680

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-tyrosine kinase 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 30.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13882 (Residues: 185-446; Coverage: 58%)
Gene names: BRK, PTK6
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 1N1 4 x 1N1
Ligand GOL 12 x GOL
1 modified residue:
Ligand CXM 4 x CXM

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 49.881Å b: 77.018Å c: 87.413Å
α: 81.36° β: 75.76° γ: 74.92°
R-values:
R R work R free
0.208 0.206 0.26
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Putting the BRK on breast cancer: From molecular target to therapeutics.
Ang et al. (2021)
2 more

5 mentions without citation

Co-crystal structures of PTK6: With Dasatinib at 2.24 Å, with novel imidazo[1,2-a]pyrazin-8-amine derivative inhibitor at 1.70 Å resolution.
Thakur et al. (2017)
4 more