Function and Biology Details
Reaction catalysed:
Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169740 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 46.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Length: 423 amino acids
Theoretical weight: 46.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
- Canonical:
Q02821 (Residues: 88-510; Coverage: 78%)
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Ubiquitin-like-specific protease 1
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
Length: 23 amino acids
Theoretical weight: 2.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
- Canonical: Q02724 (Residues: 150-172; Coverage: 4%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-17A
Spacegroup:
P212121
Expression system: Escherichia coli
