› 5h43

X-ray diffraction
2.3Å resolution

Structural and mechanistical studies of the nuclear import by Importin-alpha

Released:
DOI: 10.2210/pdb5h43/pdb
PDB entry 5h43 coloured by chain, front view.
PDB entry 5h43 coloured by chain, side view.
PDB entry 5h43 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the nuclear import of the histone acetyltransferase males-absent-on-the-first by importin α1.
Zheng W, Wang R, Liu X, Tian S, Yao B, Chen A, Jin S, Li Y
Traffic 19 19-28 (2018)
PMID: 28991411

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156497 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 46.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52292 (Residues: 70-497; Coverage: 81%)
Gene names: KPNA2, RCH1, SRP1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Histone acetyltransferase KAT8 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H7Z6 (Residues: 140-149; Coverage: 2%)
Gene names: KAT8, MOF, MYST1, PP7073
Histone acetyltransferase KAT8 Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H7Z6 (Residues: 128-142; Coverage: 3%)
Gene names: KAT8, MOF, MYST1, PP7073

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 82.222Å b: 82.222Å c: 169.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Comparative study of the interactions between fungal transcription factor nuclear localization sequences with mammalian and fungal importin-alpha.
Bernardes et al. (2020)
1 more

1 mention without citation

Elucidation of the inhibitory activity of ivermectin with host nuclear importin α and several SARS-CoV-2 targets.
Bello M. (2022)