› 5h65

X-ray diffraction
2.1Å resolution

Crystal structure of human POT1 and TPP1

Released:
DOI: 10.2210/pdb5h65/pdb
PDB entry 5h65 coloured by chain, front view.
PDB entry 5h65 coloured by chain, side view.
PDB entry 5h65 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into POT1-TPP1 interaction and POT1 C-terminal mutations in human cancer.
Chen C, Gu P, Wu J, Chen X, Niu S, Sun H, Wu L, Li N, Peng J, Shi S, Fan C, Huang M, Wong CC, Gong Q, Kumar-Sinha C, Zhang R, Pusztai L, Rai R, Chang S, Lei M
Nat Commun 8 14929 (2017)
PMID: 28393832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188359 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NUX5 (Residues: 341-634; Coverage: 46%)
Gene name: POT1
Sequence domains: Protection of telomeres protein 1 (POT1), C-terminal insertion domain
Adrenocortical dysplasia protein homolog Chain: B
Molecule details ›
Chain: B
Length: 53 amino acids
Theoretical weight: 5.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96AP0 (Residues: 180-230; Coverage: 11%)
Gene names: ACD, PIP1, PTOP, TINT1, TPP1

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 49.231Å b: 87.824Å c: 87.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Shelterin-Mediated Telomere Protection.
de Lange T. (2018)
16 more

10 mentions without citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
9 more