› 5h78

X-ray diffraction
2Å resolution

Crystal structure of the PKA-DHR14 fusion protein

Released:
DOI: 10.2210/pdb5h78/pdb
PDB entry 5h78 coloured by chain, front view.
PDB entry 5h78 coloured by chain, side view.
PDB entry 5h78 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 22.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13861 (Residues: 5-49; Coverage: 11%)
Gene names: PKR2, PRKAR2, PRKAR2A
Sequence domains: Regulatory subunit of type II PKA R-subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 179.234Å b: 96.539Å c: 39.351Å
α: 90° β: 89.9° γ: 90°
R-values:
R R work R free
0.182 0.181 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles

1 mention without citation

A simplified machine learning model utilizing platelet-related genes for predicting poor prognosis in sepsis.
Diao et al. (2023)