› 5h7v

X-ray diffraction
3.82Å resolution

Structure of full-length extracellular domain of HAI-1 at pH 4.6

Released:
DOI: 10.2210/pdb5h7v/pdb
PDB entry 5h7v coloured by chain, front view.
PDB entry 5h7v coloured by chain, side view.
PDB entry 5h7v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of a multidomain protease inhibitor (HAI-1) reveals the mechanism of its auto-inhibition.
Liu M, Yuan C, Jensen JK, Zhao B, Jiang Y, Jiang L, Huang M
J Biol Chem 292 8412-8423 (2017)
PMID: 28348076

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kunitz-type protease inhibitor 1 Chain: A
Molecule details ›
Chain: A
Length: 421 amino acids
Theoretical weight: 47.3 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O43278 (Residues: 36-457; Coverage: 82%)
Gene names: HAI1, SPINT1, UNQ223/PRO256
Sequence domains:
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P41212
Unit cell:
a: 95.62Å b: 95.62Å c: 124.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.253 0.328
Expression system: Drosophila melanogaster

Quick links

Citations

1 review citation

Trypsin-Like Proteases and Their Role in Muco-Obstructive Lung Diseases.
Carroll et al. (2021)
4 other articles

2 mentions without citation

Priming of SARS-CoV-2 S protein by several membrane-bound serine proteinases could explain enhanced viral infectivity and systemic COVID-19 infection.
Fuentes-Prior P. (2021)
1 more