› 5h8o

X-ray diffraction
4.21Å resolution

Crystal structure of an ASC-binding nanobody in complex with the CARD domain of ASC

Released:
DOI: 10.2210/pdb5h8o/pdb
PDB entry 5h8o coloured by chain, front view.
PDB entry 5h8o coloured by chain, side view.
PDB entry 5h8o coloured by chain, top view.
Source organisms:
Primary publication:
A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly.
Schmidt FI, Lu A, Chen JW, Ruan J, Tang C, Wu H, Ploegh HL
J Exp Med 213 771-90 (2016)
PMID: 27069117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212836 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
VHH nanobody Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.25 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Apoptosis-associated speck-like protein containing a CARD Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULZ3 (Residues: 115-195; Coverage: 42%)
Gene names: ASC, CARD5, PYCARD, TMS1
Sequence domains: Caspase recruitment domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P2
Unit cell:
a: 53.625Å b: 30.996Å c: 79.244Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.303 0.3 0.356
Expression system: Escherichia coli

Quick links

Citations

39 review citations

The NLRP3 inflammasome: molecular activation and regulation to therapeutics.
Swanson et al. (2019)
38 more

2 mentions without citation

A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly.
Schmidt et al. (2016)
1 more