› 5h8x

X-ray diffraction
1.3Å resolution

Crystal structure of the complex MMP-8/BF471 (catechol inhibitor)

Released:
DOI: 10.2210/pdb5h8x/pdb
PDB entry 5h8x coloured by chain, front view.
PDB entry 5h8x coloured by chain, side view.
PDB entry 5h8x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catechol-based matrix metalloproteinase inhibitors with additional antioxidative activity.
Tauro M, Laghezza A, Loiodice F, Piemontese L, Caradonna A, Capelli D, Montanari R, Pochetti G, Di Pizio A, Agamennone M, Campestre C, Tortorella P
J Enzyme Inhib Med Chem 31 25-37 (2016)
PMID: 27556138

Function and Biology Details

Reaction catalysed: 
Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil collagenase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22894 (Residues: 100-262; Coverage: 37%)
Gene names: CLG1, MMP8
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

5 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
Ligand 5XT 1 x 5XT
Ligand 7FY 1 x 7FY
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 32.91Å b: 68.46Å c: 68.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biomolecular basis of matrix metallo proteinase-9 activity.
Swetha et al. (2018)
7 other articles

7 mentions without citation

Comparative Metabolic Study of Tamarindus indica L.'s Various Organs Based on GC/MS Analysis, In Silico and In Vitro Anti-Inflammatory and Wound Healing Activities.
Aly et al. (2022)
6 more