› 5h9a

X-ray diffraction
1.38Å resolution

Crystal structure of the Apo form of human cellular retinol binding protein 1

Released:
DOI: 10.2210/pdb5h9a/pdb
PDB entry 5h9a coloured by chain, front view.
PDB entry 5h9a coloured by chain, side view.
PDB entry 5h9a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures.
Silvaroli JA, Arne JM, Chelstowska S, Kiser PD, Banerjee S, Golczak M
J Biol Chem 291 8528-40 (2016)
PMID: 26900151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09455 (Residues: 2-135; Coverage: 99%)
Gene names: CRBP1, RBP1
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 37.35Å b: 37.96Å c: 94.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.171
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Cellular retinoid binding-proteins, CRBP, CRABP, FABP5: Effects on retinoid metabolism, function and related diseases.
Napoli JL. (2017)
7 more

2 mentions without citation

Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures.
Silvaroli et al. (2016)
1 more