› 5h9b

X-ray diffraction
2.25Å resolution

Drosophila CaMKII-wt in complex with a fragment of the Eag potassium channel and Mg2+/AMPPN

Released:
DOI: 10.2210/pdb5h9b/pdb
PDB entry 5h9b coloured by chain, front view.
PDB entry 5h9b coloured by chain, side view.
PDB entry 5h9b coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The Interaction between the Drosophila EAG Potassium Channel and the Protein Kinase CaMKII Involves an Extensive Interface at the Active Site of the Kinase.
Castro-Rodrigues AF, Zhao Y, Fonseca F, Gabant G, Cadene M, Robertson GA, Morais-Cabral JH
J Mol Biol 430 5029-5049 (2018)
PMID: 30381148

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169397 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium/calmodulin-dependent protein kinase type II alpha chain Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 32.18 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00168 (Residues: 1-283; Coverage: 53%)
Gene names: CG18069, CaM, CaMKII
Sequence domains: Protein kinase domain
Structure domains:
Potassium voltage-gated channel protein eag Chain: B
Molecule details ›
Chain: B
Length: 54 amino acids
Theoretical weight: 5.41 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02280 (Residues: 770-820; Coverage: 4%)
Gene names: CG10952, eag

Ligands and Environments

3 bound ligands:
Ligand AN2 1 x AN2
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 35.823Å b: 59.127Å c: 70.339Å
α: 90° β: 99.02° γ: 90°
R-values:
R R work R free
0.204 0.203 0.222
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

1 review citation

CaMKII: a central molecular organizer of synaptic plasticity, learning and memory.
Yasuda et al. (2022)
1 other article

4 mentions without citation

Evolution of protein kinase substrate recognition at the active site.
Bradley et al. (2019)
3 more