› 5hbf

X-ray diffraction
1.95Å resolution

Crystal structure of human full-length chitotriosidase (CHIT1)

Released:
DOI: 10.2210/pdb5hbf/pdb
PDB entry 5hbf coloured by chain, front view.
PDB entry 5hbf coloured by chain, side view.
PDB entry 5hbf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-Ray Crystal Structure of the Full Length Human Chitotriosidase (CHIT1) Reveals Features of Its Chitin Binding Domain.
Fadel F, Zhao Y, Cousido-Siah A, Ruiz FX, Mitschler A, Podjarny A
PLoS One 11 e0154190 (2016)
PMID: 27111557

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-171609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitotriosidase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 53.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13231 (Residues: 1-466; Coverage: 100%)
Gene name: CHIT1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 51.143Å b: 106.661Å c: 85.67Å
α: 90° β: 107.13° γ: 90°
R-values:
R R work R free
0.206 0.205 0.245
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Chitin and chitinase: Role in pathogenicity, allergenicity and health.
Patel et al. (2017)
4 more

5 mentions without citation

Structure of the Cladosporium fulvum Avr4 effector in complex with (GlcNAc)6 reveals the ligand-binding mechanism and uncouples its intrinsic function from recognition by the Cf-4 resistance protein.
Hurlburt et al. (2018)
4 more