› 5hbs

X-ray diffraction
0.89Å resolution

Crystal structure of human cellular retinol binding protein 1 in complex with all-trans-retinol at 0.89 angstrom.

Released:
DOI: 10.2210/pdb5hbs/pdb
PDB entry 5hbs coloured by chain, front view.
PDB entry 5hbs coloured by chain, side view.
PDB entry 5hbs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures.
Silvaroli JA, Arne JM, Chelstowska S, Kiser PD, Banerjee S, Golczak M
J Biol Chem 291 8528-40 (2016)
PMID: 26900151

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09455 (Residues: 2-135; Coverage: 99%)
Gene names: CRBP1, RBP1
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand RTL 1 x RTL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 37.217Å b: 49.769Å c: 73.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.125 0.133
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Molecular Basis for Vitamin A Uptake and Storage in Vertebrates.
Chelstowska et al. (2016)
5 more

6 mentions without citation

Cellular retinoid binding-proteins, CRBP, CRABP, FABP5: Effects on retinoid metabolism, function and related diseases.
Napoli JL. (2017)
5 more