› 5hce

X-ray diffraction
3.12Å resolution

Ternary complex of human Complement C5 with Ornithodoros moubata OmCI and Rhipicephalus appendiculatus RaCI1

Released:
DOI: 10.2210/pdb5hce/pdb
PDB entry 5hce coloured by chain, front view.
PDB entry 5hce coloured by chain, side view.
PDB entry 5hce coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for therapeutic inhibition of complement C5.
Jore MM, Johnson S, Sheppard D, Barber NM, Li YI, Nunn MA, Elmlund H, Lea SM
Nat Struct Mol Biol 23 378-86 (2016)
PMID: 27018802

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-103003 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Complement C5 beta chain Chain: B
Molecule details ›
Chain: B
Length: 656 amino acids
Theoretical weight: 73.52 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01031 (Residues: 19-674; Coverage: 40%)
Gene names: C5, CPAMD4
Sequence domains:
Complement C5 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 998 amino acids
Theoretical weight: 112.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01031 (Residues: 679-1676; Coverage: 60%)
Gene names: C5, CPAMD4
Sequence domains:
Complement inhibitor Chain: C
Molecule details ›
Chain: C
Length: 165 amino acids
Theoretical weight: 18.65 KDa
Source organism: Ornithodoros moubata
Expression system: Kluyveromyces lactis
UniProt:
  • Canonical: Q5YD59 (Residues: 1-1, 19-168; Coverage: 89%)
Structure domains: Lipocalin
Complement inhibitor RaCI1 Chain: D
Molecule details ›
Chain: D
Length: 81 amino acids
Theoretical weight: 8.58 KDa
Source organism: Rhipicephalus appendiculatus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A141SFN4 (Residues: 21-98; Coverage: 100%)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CYS 1 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 105.26Å b: 140.725Å c: 210.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.267 0.281
Expression systems:
  • Kluyveromyces lactis
  • Escherichia coli K-12

Quick links

Citations

23 review citations

Protection of host cells by complement regulators.
Schmidt et al. (2016)
22 more

5 mentions without citation

Structural basis for therapeutic inhibition of complement C5.
Jore et al. (2016)
4 more