› 5hem

X-ray diffraction
1.65Å resolution

Crystal structure of the N-terminus D161Y bromodomain mutant of human BRD2

Released:
DOI: 10.2210/pdb5hem/pdb
PDB entry 5hem coloured by chain, front view.
PDB entry 5hem coloured by chain, side view.
PDB entry 5hem coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tallant C, Lori C, Pasquo A, Chiaraluce R, Consalvi V, Newman JA, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Knapp S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25440 (Residues: 71-194; Coverage: 16%)
Gene names: BRD2, KIAA9001, RING3
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand ACT 2 x ACT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 68.73Å b: 68.73Å c: 113.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.199
Expression system: Escherichia coli

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